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聯(lián)系人：楊燕燕
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技術文章
GDS MPX Top 7 STEC
點擊次數(shù)：28 發(fā)布時間：2024/8/7 17:28:07
一般描述
Assurance® GDS is an advanced genetic detection system for food pathogens. The MPX Top 7 STEC is an automated nucleic acid amplification system for the detection of E. coli O157:H7 ａnd the "Top Six" non-O157 Shiga Toxigenic E. coli (STEC). The Top Six non-O157 STEC are defined as E. coli belonging to serogroups O103, O111, O121, O145, O26, or O45 that possess both the eae gene ａnd at least one of the shiga toxin genes (stx1 or stx2). Assurance® GDS MPX Top 7 STEC utilizes a proprietary PickPen® IMS-based sample preparation procedure to quickly ａnd effectively capture organisms belonging to the 7 specific Top STEC O serogroups prior to genetic analysis for the associated pathogenicity genes.
應用
Assurance® GDS MPX Top 7 STEC is suitable for the detection of E. coli O157:H7 ａnd the “Top Six” non-O157 Shiga Toxigenic E. coli (STEC) in foods, ingredients, ａnd environmental samples.
特點和優(yōu)勢
- Following sample preparation with IMS against the Top 7 O groups, samples are analyzed with a single multiplex assay for the presence of (eae), stx1, and stx2
- Assurance® GDS MPX Top 7 STEC assay includes a separate target ａnd differentiated result for E. coli O157:H7
- Single-step 10-18 h enrichment in mEHEC®, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, ａnd Top STEC
組分
- MPX Top STEC Amplification Tubes
- Top 7 STEC Concentration ReagentResuspension Buffer TqTop STEC Wash Solution
相容性
BioControl GDS
測試方法
PCR, Immunomagnetic Separation (IMS)
法律信息
AOAC is a registered trademark of AOAC International
Assurance is a registered trademark of Merck KGaA, Darmstadt, Germany
Assurance GDS is a registered trademark of BioControl Systems, Inc.
PICKPEN is a registered trademark of Merck KGaA, Darmstadt, Germany
mEHEC is a registered trademark of Merck KGaA, Darmstadt, Germany
原創(chuàng)作者：上海篤瑪生物科技有限公司
