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技術文章
Viral RNA Extraction Buffer
點擊次數：19 發(fā)布時間：2024/8/7 17:21:10
一般描述
The Viral RNA Extraction Buffer has been optimized to provide rapid, room-temperature lysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles in saliva. The buffer comes as a 4x working solution that can be mixed with virus-containing samples in a qRT-PCR plate. Upon 5 minute incubation at room temperature, viral particles are lysed ａnd RNA is stabilized such that samples are ready for direct analysis via RT-qPCR with no additional cleanup step. Removal of precipitates in saliva via centrifugation prior to testing is recommended for optimal sensitivity.
The Viral RNA Extraction Buffer is broadly compatible with most nucleic acid detection methods ａnd does not reduce signal when used at working concentration in qRT-PCR. qRT-PCR compatibility has been demonstrated in a 4-fluor multiplex setting consisting of Cy5, TxRed, FAM, ａnd HEX.RT-qPCR compatibility has been verified with several one-step RT-qPCR kits:
- Quantitative RT-PCR ReadyMix™ (Sigma-Aldrich, catalog QR0200)
- KAPA PROBE FAST One-Step Universal (Sigma-Aldrich, catalog KK4752)
- KiCqStart® One-Step Probe RT-qPCR ReadyMix™ (Sigma-Aldrich, catalog KCQS07)
- TaqPathTM 1-Step Multiplex Master Mix (Thermo Fisher, catalog A28525)
The Viral RNA Extraction Buffer is also compatible with colorimetric LAMP assays.
特點和優(yōu)勢
- Extracted viral RNA can be used for detection without cleanup
- Directly compatible with quantitative reverse transcription polymerase chain reaction (qRT-PCR) ａnd loop mediated isothermal amplification(LAMP) assays
- Ideal for enveloped RNA viruses in saliva, saline, Amies medium, ａnd viral transport medium
- Highly sensitive ａnd faster than other leading methods, lysis by Proteinase K ａnd heating
法律信息
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
原創(chuàng)作者：上海篤瑪生物科技有限公司
