推薦：HiCrome™ ECC 選擇性培養(yǎng)基@2024已更新

別名:ECC 選擇性培養(yǎng)基 HiCrome™, 大腸桿菌-大腸菌選擇性培養(yǎng)基 HiCrome™

MDL編號:MFCD01634645

NACRES:NA.85

屬性
無菌性
non-sterile

質(zhì)量水平
200

形式
powder

保質(zhì)期
limited shelf life, expiry date on the label

組成
agar, 10.0 g/L
casein enzymic hydrolysate, 3.3 g/L
chromogenic mixture, 0.43 g/L
disodium hydrogen phosphate, 1 g/L
peptone, special, 6.0 g/L
sodium chloride, 2 g/L
sodium dihydrogen phosphate, 0.6 g/L
sodium pyruvate, 1 g/L
sorbitol, 1 g/L
Tergitol™
7, 0.15 g/L
tryphan, 1 g/L

manufacturer/tradename
NutriSelect® Plus

終pH
6.8±0.2 (25 °C)

application(s)
agriculture
bioburden testing
environmental
food ａnd beverages
water monitoring
microbiology

適用性
selective ａnd differential for Citrobacter spp.
selective ａnd differential for Enterobacter spp.
selective ａnd differential for Escherichia coli
selective ａnd differential for Salmonella spp.
selective ａnd differential for Shigella spp.
selective ａnd differential for coliforms
selective ａnd differential for enterobacteriaceae

說明
應(yīng)用
HiCrome™ ECC 選擇性瓊脂是一種選擇性培養(yǎng)基，推薦用于對水和食物樣品中的大腸桿菌和大腸菌群進行同時檢測。該培養(yǎng)基中的成分甚至有助于亞致死性損傷的大腸菌迅速生長，Tergitol™ 可以抑制革蘭氏陽性和某些革蘭氏陰性細菌，而不影響大腸菌。顯色混合物包含兩種顯色底物：Salmon-GAL 和 X-葡萄糖苷酸。大腸菌產(chǎn)生的 β-D-半乳糖苷酶可裂解 Salmon-GAL，使大腸菌菌落呈現(xiàn)鮮肉色至紅色。大腸桿菌產(chǎn)生的 β-D-葡萄糖醛酸苷酶則可裂解 X-葡萄糖苷酸。由于 Salmon-GAL 和 X-葡萄糖苷酸均被裂解，因此大腸桿菌形成深藍色到紫色的菌落。添加色氨酸可增進吲哚反應(yīng)。為了確認大腸桿菌，可將一滴柯氏試劑（目錄號 60983）滴在深藍色到紫色的菌落上。形成櫻桃紅色則說明反應(yīng)呈陽性。
制備說明
Suspend 26.5 g in 1 litre distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas ａnd Aeromonas species add 5 mg Cefsulodin (Cat. No. 22126) for surface ａnd pour plate method ａnd 10 mg Cefsulodin for membrane filter technique. Mix well.
重懸
Suspend 26.5 g in 1 liter of distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas ａnd Aeromonas species, add 5 mg of cefsulodin for surface ａnd pour plate method ａnd 10 mg of cefsulodin for membrane filter technique. Mix well.
附注
We offer two media types: the superior granulated GranuCult® ａnd the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

IF<5，贈送價值 500元禮品卡
5 ≤IF≤10，贈送價值 1000元禮品卡
IF≥10，贈送價值 1000元禮品卡+任意 1 款 ValukineTM 試劑盒


DUMABIO ELISA試劑盒文獻征集獎勵活動

為感謝各位老師對篤瑪品牌的支持與厚愛，凡購買DUMABIO ELISA試劑盒，在SCI期刊雜志發(fā)表文中注明ELISA試劑盒采購上海篤瑪生物科技有限公司（Shanghai DuMa Biotechnology Co., Ltd.）

Shanghai DuMa Biotechnology Co., Ltd. 

發(fā)表刊物        影響因子           獎勵金額
SCI期刊雜志      1.0≤1F＜3.0          200元
SCI期刊雜志      3.0≤1F＜6.0          400元
SCI期刊雜志      6.0≤1F＜10.0         600元
SCI期刊雜志      1F≥10                1000元