推薦：S-Gal®/LB Agar Blend@2024已更新

NACRES:NA.85

屬性
等
for molecular biology

質量水平
200

無菌性
non-sterile

形式
powder

technique(s)
microbiological culture: suitable

適用性
suitable for β-galactosidase test
nonselective for Escherichia coli
nonselective for coliforms

application(s)
food ａnd beverages
microbiology

儲存溫度
room temp

說明
一般描述
S-Gal® (sodium salt) is an autoclavable, water-soluble, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA ｉnsert in bacteria growing on agar plates. S-gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
應用
S-Gal®/LB Agar Blend has been used for the identification of recombinant bacterial colonies.[1][2][3]
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® (sodium salt) is water-soluble, autoclavable ａnd can be added to bacterial broth containing agar prior to autoclaving.
特點和優(yōu)勢
More intense color contrast than X-gal
Water-soluble ａnd autoclavable for easiest use
Convenient, pre-mixed media
組分
Ingredients (g/L)
Tryptone, 10
Yeast extract, 5
Sodium chloride, 10
Agar, 12
S-Gal, 0.3
Ferric ammonium citrate, 0.5
IPTG, 0.03
原理
When S-Gal® is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® ａnd ferric ion turn an intense black color, allowing for easy differentiation between lac+ ａnd lac- colonies.
制備說明
Suspend contents of one packet in 500 ml distilled or deionized water. Sterilize by autoclaving for 15 to 20 minutes at 121-124°C. For microwaving, heat suspended mix until initial boiling. Mix well. Heat for short intervals with mixing until agar component is in solution. Do not allow boiling for extended periods of time. Antibiotics should be added following autoclaving or microwaving, after cooling to 48-52°C.
其他說明
The ferric or Fe3+ ion is required for color development ａnd must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.

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發(fā)表刊物        影響因子           獎勵金額
SCI期刊雜志      1.0≤1F＜3.0          200元
SCI期刊雜志      3.0≤1F＜6.0          400元
SCI期刊雜志      6.0≤1F＜10.0         600元
SCI期刊雜志      1F≥10                1000元