LB Broth with agar (Luria low salt)

別名:Luria Agar Base (Miller’s Modification)

屬性
等
for molecular biology

質(zhì)量水平
200

無菌性
non-sterile

形式
powder

組成
Agar, 15 g/L
NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

technique(s)
microbiological culture: suitable

pH值（酸堿度）
6.8-7.2(3.05% solution)

application(s)
food ａnd beverages
microbiology

儲(chǔ)存溫度
room temp

適用性
nonselective for Escherichia coli
nonselective for coliforms

說明
一般描述
Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, ａnd carbohydrates. The addition of agar provides a solid medium for microbial growth.
應(yīng)用
LB Broth with agar (Luria low salt) has been used:
for culturing E. coli DH10B cells. [1]
in the isolation of diazotrophic bacteria. [2]
for transformation of competent E. coli strain Stbl3 for cloning. [3]
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production ａnd production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
特點(diǎn)和優(yōu)勢
Luria′s low salt LB powder with agar provides:
Easy scale-up using larger package sizes
A budget-friendly alternative to pre-poured plates
Standard formulation
制備說明
1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.

IF<5，贈(zèng)送價(jià)值 500元禮品卡
5 ≤IF≤10，贈(zèng)送價(jià)值 1000元禮品卡
IF≥10，贈(zèng)送價(jià)值 1000元禮品卡+任意 1 款 ValukineTM 試劑盒


DUMABIO ELISA試劑盒文獻(xiàn)征集獎(jiǎng)勵(lì)活動(dòng)

為感謝各位老師對篤瑪品牌的支持與厚愛，凡購買DUMABIO ELISA試劑盒，在SCI期刊雜志發(fā)表文中注明ELISA試劑盒采購上海篤瑪生物科技有限公司（Shanghai DuMa Biotechnology Co., Ltd.）

Shanghai DuMa Biotechnology Co., Ltd. 

發(fā)表刊物        影響因子           獎(jiǎng)勵(lì)金額
SCI期刊雜志      1.0≤1F＜3.0          200元
SCI期刊雜志      3.0≤1F＜6.0          400元
SCI期刊雜志      6.0≤1F＜10.0         600元
SCI期刊雜志      1F≥10                1000元