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聯(lián)系我們
聯(lián)系人:楊燕燕
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技術文章
推薦:Bactident™ Kovac′s Indole reagent@2024已更新
推薦:Bactident™ Kovac′s Indole reagent@2024已更新
別名:4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac, Indole reagent, Indole test reagent
屬性
質量水平
100
形式
liquid (ready-to-use)
保質期
210 days
特點
ready-to-use
組成
(n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)
包裝
pkg of (1 x 30 ml, Plastic bottle)
technique(s)
microbe id | specific enzyme detection: suitable
pH值(酸堿度)
<1 (20 °C in H2O)
轉變溫度
flash point 36 °C
密度
0.92 g/cm3 at 20 °C
application(s)
environmental
food and beverages
相容性
Bactident™ E. coli 1.13303
儲存溫度
2-8°C
適用性
Enterobacter spp.
Escherichia coli
說明
一般描述
Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryphan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryphan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryphan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.
應用
Bactident™ Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.
聯(lián)系
Replaces: 1.11350.0007
分析說明
Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryphan broth.
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Shanghai DuMa Biotechnology Co., Ltd.
發(fā)表刊物 影響因子 獎勵金額
SCI期刊雜志 1.0≤1F<3.0 200元
SCI期刊雜志 3.0≤1F<6.0 400元
SCI期刊雜志 6.0≤1F<10.0 600元
SCI期刊雜志 1F≥10 1000元
原創(chuàng)作者:上海篤瑪生物科技有限公司