推薦：KOVACS′ indole reagent

別名:4-(Dimethylamino)benzaldehyde solution, Indole reagent, Kovac′s reagent, Kovac′s reagent for indoles

屬性
質(zhì)量水平
200

形式
liquid

包裝
pkg of 100 mL

儲存條件
(No metal containers. Keep the container tightly closed in a dry ａnd well-ventilated place. Keep away from heat ａnd sources of ignition)

technique(s)
microbe id | specific enzyme detection: suitable

顏色
yellow

pH值（酸堿度）
<1 (20 °C in H2O)

轉(zhuǎn)變溫度
flash point 36 °C

密度
0.92 g/cm3 at 20 °C

application(s)
microbiology

儲存溫度
2-8°C

適用性
Escherichia coli
enterovirulent E. coli (EEC) spp.

說明
一般描述
Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, ａnd 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryphan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryphan to give pyruvic acid, ammonia, ａnd indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryphan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.
應(yīng)用
Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food ａnd beverages, ａnd environmental samples. This is suitable for the identification of E. coli.
分析說明
Indole formation (Escherichia coli ATCC 25922 (WDCM 00013)): +
Indole formation (Proteus vulgaris ATCC 8427): +
Indole formation (Enterobacter aerogenes ATCC 13048): -
Tested in DEV-Tryphan broth art. no. 1.10694. (after incubation 24 hrs.; 35 °C aerobic).
其他說明
The reagent solution must be stored in the dark in the refrigerator, otherwise it may turn brown ａnd cannot be used.

IF<5，贈送價(jià)值 500元禮品卡
5 ≤IF≤10，贈送價(jià)值 1000元禮品卡
IF≥10，贈送價(jià)值 1000元禮品卡+任意 1 款 ValukineTM 試劑盒


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發(fā)表刊物        影響因子           獎勵金額
SCI期刊雜志      1.0≤1F＜3.0          200元
SCI期刊雜志      3.0≤1F＜6.0          400元
SCI期刊雜志      6.0≤1F＜10.0         600元
SCI期刊雜志      1F≥10                1000元