別名:硫氰酸亞脲, 硫氰酸胍

線性分子式:NH2C(=NH)NH2 · HSCN

CAS號(hào):593-84-0

​
分子量:118.16

Beilstein:3563461

EC 號(hào):209-812-1

MDL編號(hào):MFCD00013027

PubChem化學(xué)物質(zhì)編號(hào):24895367

NACRES:NA.31

生物來(lái)源
synthetic (organic)

質(zhì)量水平
200

等
for molecular biology

檢測(cè)方案
≥99%

形式
powder or crystals

儲(chǔ)存條件
(Tightly closed. Dry. )

technique(s)
RNA extraction: suitable

顏色
colorless to white,


mp
120-122 °C (lit.)

溶解性
water: 100 mg/mL, clear, colorless

密度
1.29 g/cm3 at 20 °C (68 °F)

痕量陽(yáng)離子
Ba: ≤5 ppm
Fe: ≤5 ppm
Na: ≤0.5%
heavy metals (as Pb): ≤5 ppm

application(s)
agriculture

異質(zhì)活性
DNase, RNase, detected

SMILES string
SC#N.NC(N)=N

InChI
1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key
ZJYYHGLJYGJLLN-UHFFFAOYSA-N

一般描述
Guanidine thiocyanate, or guanidinium rhodanide, is a versatile chemical compound widely used in molecular biology ａnd biochemical research. Acting as a chaotropic agent, it disrupts cell ａnd organelle membranes, facilitating cell lysis ａnd organelle isolation. Notably, it serves as a potent protein denaturant, surpassing guanidine hydrochloride in unfolding proteins. This property is valuable for purifying nucleic acids from crude cell lysates, effectively separating nucleic acids from denatured proteins.

In nucleic acid purification, guanidine thiocyanate is effective in inactivating DNases ａnd RNases, preventing nucleic acid degradation ａnd ensuring favorable yields. Its dual denaturing ａnd enzyme-inhibiting properties make it crucial for DNA ａnd RNA studies. Beyond nucleic acid isolation, guanidine thiocyanate is used for cell lysis ａnd virus particle inactivation during RNA ａnd DNA extractions. It not only disrupts membranes but also denatures RNase ａnd DNase enzymes, protecting extracted nucleic acids from enzymatic degradation. The versatility of guanidine thiocyanate extends to molecular biology techniques like PCR, Northern blotting, ａnd Southern blotting. It serves as a vital reagent in these methods, contributing to the successful analysis ａnd amplification of nucleic acids.
應(yīng)用
胍硫氰酸鹽已用于：
RNA分離之裂解大腸腺癌細(xì)胞系的[1]
作為三重線粒體DNA (mtDNA) qPCR反應(yīng)體系中的PCR抑制劑[2]
用于從腦脊液（CSF）中提取DNA的qPCR分析[3]

離液劑和 變性劑;溶解細(xì)胞。
生化/生理作用
胍硫氰酸鹽是一種變性劑，常用于RNA分離。它可用作全血樣品的儲(chǔ)存緩沖液。[4]胍硫氰酸鹽可滅活核酸酶，是儲(chǔ)存和冷凍糞便樣本進(jìn)行DNA研究的理想選擇。[5]它可與苯酚?氯仿聯(lián)用提取RNA。[6]
特點(diǎn)和優(yōu)勢(shì)
Highly versatile surfactant for your cell biology ａnd biochemical research
Suitable for RNA extraction in sensitive molecular biology applications
Free from Dnase ａnd Rnase
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

免責(zé)聲明
試劑盒僅供研究使用，不得用于臨床實(shí)驗(yàn)或生物體實(shí)驗(yàn)，否則所產(chǎn)生的一切后果，由實(shí)驗(yàn)者承擔(dān)， 本公司概不負(fù)責(zé)。
嚴(yán)格按照說(shuō)明書(shū)操作，不同批號(hào)不可交換使用，實(shí)驗(yàn)者違反說(shuō)明書(shū)操作，后果由實(shí)驗(yàn)者承擔(dān)。



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科研ELISA是上海篤瑪生物的主營(yíng)產(chǎn)品，科研ELISA欄目提供該類(lèi)目產(chǎn)品說(shuō)明書(shū)以及提供產(chǎn)品報(bào)價(jià)與網(wǎng)上訂購(gòu)。

上海篤瑪生物科技有限公司經(jīng)過(guò)數(shù)年的努力發(fā)展已迅速成為集產(chǎn)品研發(fā)、生產(chǎn)、經(jīng)營(yíng)為一體的業(yè)化生物工程公司。公司具有完善的實(shí)驗(yàn)技術(shù)開(kāi)發(fā)平臺(tái)，成熟的抗原、抗體研發(fā)系統(tǒng)，熟練掌握各種酶聯(lián)技術(shù)，結(jié)合公司的研發(fā)團(tuán)隊(duì)，可以有效的保證公司產(chǎn)品強(qiáng)的穩(wěn)定性和高的準(zhǔn)確性，同時(shí)又具有競(jìng)爭(zhēng)力的價(jià)格。

公司目主要提供生化試劑、分子生物學(xué)試劑及試劑盒，各種抗體及免疫學(xué)試劑盒、實(shí)驗(yàn)耗材等多門(mén)類(lèi)上萬(wàn)種產(chǎn)品，產(chǎn)品涉及分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)等生命科學(xué)的各個(gè)域。

原創(chuàng)作者：上海篤瑪生物科技有限公司