名稱： C1酯酶抑制劑 C1 Esterase Inhibitor (C1-INH)

• 檢測方法

  Colorimetric

• 度

  批次內(nèi)

  樣品	n	Mean	SD	CV%
  Plasma 1	20			3.7%
  Plasma 2	20			4.1%
  Plasma 3	20			3.5%

  批次間

  樣品	n	Mean	SD	CV%
  Plasma 1	20			9.8%
  Plasma 2	20			9.2%
  Plasma 3	20			8.4%

• 樣品類型

  Cell culture supernatant, Saliva, Urine, Serum, Plasma, Cerebral Spinal Fluid

• 檢測類型

  Sandwich (quantitative)

• 靈敏度

  0.26 ng/ml

• 范圍

  0.156 ng/ml - 10 ng/ml

• 回收率

  96 %

• 檢測時間

  4h 00m

• 實驗步驟

  Multiple steps standard assay

• 種屬反應性

  與反應: Human
  不與反應: Mouse, Rat, Rabbit, Cow, Dog, Pig

測定

• 產(chǎn)品概述

  The Human Complement Factor P (CFP) ELISA (Enzyme-Linked Immunosorbent Assay) Kit (ab222864) is designed for detection of Complement Factor P in human plasma, serum, urine, saliva, CSF, ａnd cell culture samples.

  
  

  This assay employs a quantitative sandwich enzyme immunoassay technique that measures human Complement Factor P in approximately 4 hours. A polyclonal antibody specific for human Complement Factor P has been pre-coated onto a 96-well microplate with removable strips. Complement Factor P in standards ａnd samples is sandwiched by the immobilized antibody ａnd a biotinylated polyclonal antibody specific for human Complement Factor P, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away ａnd a peroxidase enzyme substrate is added. The color development is sped ａnd the intensity of the color is measured.

  
  

  The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

遺傳學

• 說明

  Complement factor P (CFP), also known as properdin, is a plasma glycoprotein that positively regulates the alternative complement pathway convertases (C3bBb) of the innate immune system. CFP consists of 442-amino acids with a molecular weight of 53 kDa ａnd is composed of multiple identical protein subunits. The subunits bind to each other in a head to tail manner to form cyclic dimers, trimers, tetramers, pentamers, ａnd higher cyclic oligomers. It is mainly produced by neutrophils but also by monocytes, T cells, ａnd bone marrow progenitor cells. CFP binds to many microbial surfaces ａnd apoptotic cells. Complement factor P also stabilizes the C3- ａnd C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex ａnd lysis of the target cell.

原創(chuàng)作者：上海篤瑪生物科技有限公司