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一般描述
The Transcreener® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets, ａnd greatly simplifies compound ａnd inhibitor profiling across multiple target families.

The Transcreener® ADP2 FP Assay is a far-red, competitive fluorescence polarization (FP) assay based on the detection of ADP ａnd therefore is compatible with any enzyme class that produces ADP, including protein, lipid, ａnd carbohydrate kinases, ATPases, DNA helicases, carboxylases ａnd glutamine synthetase. The Transcreener® ADP2 Assay is a simple one step homogenous detection assay, ａnd is extremely flexible with regard to ATP concentration (0.1 to 1,000 μM ATP). The assay provides excellent signal at low substrate conversion, with a Z′ = 0.7 ａnd = 85 polarization shift (mP) at 10% ATP conversion using 1 μM ATP.

The Transcreener® ADP2 FP Assay was developed to follow the progress of any enzyme that produces ADP. The Transcreener® ADP Detection Mixture comprises an ADP Alexa633 Tracer bound to an ADP2 Antibody. The tracer is displaced by ADP, the invariant product generated during the enzyme reaction.The displaced tracer freely rotates leading to a decrease in fluorescence polarization. The assay uses a far red tracer to minimize interference from fluorescent compounds ａnd light scattering.

View full Transcreener® product list
數(shù)量
3010-1K = 1,000 assay, 384-well
3010-10K = 10,000 assay, 384-well
外形
Kit with buffered aqueous solutions
法律信息
Transcreener is a registered trademark of BellBrook Labs
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科研ELISA是上海篤瑪生物的主營產(chǎn)品，科研ELISA欄目提供該類目產(chǎn)品說明書以及提供產(chǎn)品報價與網(wǎng)上訂購。

上海篤瑪生物科技有限公司經(jīng)過數(shù)年的努力發(fā)展已迅速成為集產(chǎn)品研發(fā)、生產(chǎn)、經(jīng)營為一體的業(yè)化生物工程公司。公司具有完善的實驗技術開發(fā)平臺，成熟的抗原、抗體研發(fā)系統(tǒng)，熟練掌握各種酶聯(lián)技術，結合公司的研發(fā)團隊，可以有效的保證公司產(chǎn)品強的穩(wěn)定性和高的準確性，同時又具有競爭力的價格。

公司目主要提供生化試劑、分子生物學試劑及試劑盒，各種抗體及免疫學試劑盒、實驗耗材等多門類上萬種產(chǎn)品，產(chǎn)品涉及分子生物學、細胞生物學、免疫學等生命科學的各個域。

原創(chuàng)作者：上海篤瑪生物科技有限公司