Recombinant Microtubule Associated Protein 2 (MAP2)

MAP2A; MAP2B; MAP2C

[ PROPERTIES ]

Residues: Thr1457~Ser1728 (Accession # P20357),

with two N-terminal Tags, His-tag ａnd GST-tag. Host: E. coli

Subcellular Location: Cylasm, cytoskeleton.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 9.7

Predicted Molecular Mass: 61.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot ａnd store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, ａnd no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

查找：微管關聯(lián)蛋白2(MAP2)重組蛋白

Recombinant Microtubule Associated Protein 2 (MAP2)

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag ａnd GST-tag, itssequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -TVSR DEVRRKKAVYKKAELAKKSE VQAHSPSRKL ILKPAIKYTR PTHLSCVKRK TTAASGDLAQ APGAFKQAKDKVTDGISKSP EKRSSLPRPS SILPPRRGVS GDREENSFSL NSSISSARRT TRSEPIRRAGKSGTSTPTTP GSTAITPGTP PSYSSRTPGT PGTPSYPRTP GTPKSGILVP SEKKVAIIRTPPKSPATPKQ LRLINQPLPD LKNVKSKIGS TDNIKYQPKG GQVQIVTKKI DLSHVTSKCG

SLKNIRHRPG GGRVKIES

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原創(chuàng)作者：上海篤瑪生物科技有限公司