Amersham Hybond-N+ 帶正電荷尼龍膜 0.45um 30cmx3m RPN303B
帶正電荷的尼龍膜與核酸高達600μg/cm2的結(jié)合能力。建議使用放射性和非放射性的化學發(fā)光法和chemifluorescence檢測系統(tǒng)。
Amersham公司Hybond™- N +產(chǎn)品在傳統(tǒng)的Southern雜交有著出色的靈敏度。核酸樣品可能是固定的，通過紫外線照射或烘烤，雖然紫外線固定是的的重復(fù)性。 Hybond N +是不適合堿印跡和固定。

Amersham Hybond™-N+

• Positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm²¾recommended for use with radioactive or nonradioactive chemiluminescence ａnd chemifluorescence detection systems.
• Recommended for all high density membrane arrays.

Technical Information

Amersham Hybond™-N+ yields excellent sensitivity in conventional Southern blotting. Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation is best for maximum reproducibility. Hybond-N+ is not suitable for Alkali blotting ａnd fixation.

• Multiple reprobing is possible without loss of membrane integrity.
   
• QC tested in genomic Southern blots using GE Healthcare products.
   
• Special sizes available on request.

Membranes should be stored at room temperature in a clean dry atmosphere away from excessive heat, light, ａnd noxious fumes. To prevent contamination, handle membranes using gloves or blunt-ended forceps. Hybond-N+ is stable for up to 3 years. Membranes should be kept in the bags in which they are received. Performance is consistent when stored under the recommended conditions.