Microcystin微囊藻毒素-LR單克隆抗體
Monoclonal Antibody to Microcystin-LR （MC10E7）

Product Specification
 
CLONE:    MC10E7 
ISOTYPE:    Mouse IgG1 
PURITY DETAIL:    Protein G-affinity purified. 
FORMULATION:    Lyophilized. 
RECONSTITUTION:    Reconstitute with 200μl PBS containing sodium azide. 
IMMUNOGEN:    Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin. 
SPECIFICITY:    Recognizes all 4-Arg microcystins. 
APPLICATION:    ELISA （1:10＇000-1:100＇000）
Microcystin concentrations can be measured in the range of ~0.006μg/l to 0.3μg/l. The ELISA sensitivity is about 1＇000 times higher compared to UV detection. 
SHIPPING:    SHIPPED ON BLUE ICE 
LONG TERM STORAGE:    -20°C 
USE/STABILITY:    Storage at 4°C will decrease antibody activity ａnd must be avoided. 
HANDLING:    Avoid freeze/thaw cycles. After opening, prepare aliquots ａnd store at -20°C. 
 
Product Description

With a direct competitive enzyme-linked immunosorbent assay （ELISA） using antibody MC10E7, IC50 values for microcystin-LR of 0.06μg/l have been obtained. The provisional guideline value proposed by the WHO is 1 μg/l for drinking water. The detection limit for microcystin-LR is 0.006 μg/l. All microcystin variants containing an arginine at position 4 show similar IC50 values ａnd detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for MC10E7 was determined to be at least 7x1010 l/mol.
The antibody was tested for its robustness against interferences （humic acids, pH, salt content, surfactants or organic solvents） ａnd was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 ａnd 0.1μg/l were measured ａnd a mean recovery of 99.9±16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.
 
Product Specific Literature References

Highly sensitive immunoassay based on a monoclonal antibody specific for [4-arginine]microcystins: A. Zeck, et al.; Anal. Chim. Acta 441, 1 （2001）
Development of a direct competitive microcystin immunoassay of broad specificity: M.G. Weller, et al.; Anal. Sci. 17, 1445 （2001） Abstract
 
General Information
 
Microcystins are extremely toxic compounds produced by cyanobacteria （blue-green algae）, belonging to species of Microcystis, Oscillatoria, Anabaena ａnd Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans ａnd animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo（-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla）, where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid ａnd X ａnd Y are variable L-aa.
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