pam-210 —— 一臺(tái)不僅僅是用于教學(xué)的熒光儀
schreiber教授因發(fā)明pam系列調(diào)制葉綠素?zé)晒鈨x而獲得首屆光合作用協(xié)會(huì)（ispr）創(chuàng)新獎(jiǎng)

調(diào)制葉綠素?zé)晒饧夹g(shù)盡管在科研上得到了廣泛且成功的應(yīng)用，但由于儀器昂貴，因此在很長(zhǎng)一段時(shí)間里并沒(méi)能用于教學(xué)實(shí)驗(yàn)。為此，schreiber教授專(zhuān)門(mén)設(shè)計(jì)了一臺(tái)為本科生教學(xué)用的調(diào)制熒光儀——pam-210（也叫teaching-pam）。這臺(tái)儀器的基本功能與pam-2100相同，其電腦操作軟件也采用pamwin，同時(shí)還具有強(qiáng)大的單機(jī)操作功能。因此，pam-210不僅在教學(xué)應(yīng)用上取得了成功，在科研領(lǐng)域也得到了成功應(yīng)用。 schreiber教授專(zhuān)為利用pam-210進(jìn)行教學(xué)實(shí)驗(yàn)寫(xiě)了一本《葉綠素?zé)晒饣A(chǔ)實(shí)驗(yàn)指南》，我司已將該書(shū)翻譯成中文，供國(guó)內(nèi)學(xué)者參考。

特點(diǎn)
1）pam-2100的超濃縮版
2）可單機(jī)操作，可連電腦操作（操作軟件與pam-2100相同）
3）可測(cè)熒光誘導(dǎo)曲線(xiàn)的快速上升動(dòng)力學(xué)o-i-d-p相
4）可測(cè)熒光誘導(dǎo)曲線(xiàn)的慢速下降動(dòng)力學(xué)并進(jìn)行淬滅分析
5）可測(cè)光響應(yīng)曲線(xiàn)和快速光曲線(xiàn)（rlc）
6）研究對(duì)象包括高等植物和藻類(lèi)
7）專(zhuān)為針葉植物配了葉室
8）利用微光纖可檢測(cè)超小樣品的光合作用

測(cè)量參數(shù)
fo、fm、fv/fm、ft、fm’、fo’、δf/fm’、qp、qn、npq和retr等。

應(yīng)用領(lǐng)域
為本科教學(xué)實(shí)驗(yàn)設(shè)計(jì)，但具備了pam的所有基本功能，可用于植物生理學(xué)、植物生態(tài)學(xué)、農(nóng)學(xué)、園藝學(xué)、水生生物學(xué)等領(lǐng)域。

帶磁性暗適應(yīng)葉室	連接微光纖	帶特制針葉葉室	帶液體樣品室

技術(shù)參數(shù)
測(cè)量光：紅色發(fā)光二極管（led），650 nm，標(biāo)準(zhǔn)強(qiáng)度0.04 μmol m-2 s-1par；調(diào)制頻率32hz或8 khz，自動(dòng)轉(zhuǎn)換。
光化光：紅色led，665 nm，連續(xù)光強(qiáng)1800 μmol m-2 s-1par
飽和脈沖：紅色led，665 nm，連續(xù)光強(qiáng)3500 μmol m-2 s-1par
遠(yuǎn)紅光：led，730 nm，強(qiáng)度10 w m-2。
信號(hào)檢測(cè)：pin-光電二極管，帶短波截止濾光片（λ>710 nm）；選擇性鎖相放大器（設(shè)計(jì)）。
環(huán)境溫度：-5～＋45℃，在極地研究中成功應(yīng)用

部分文獻(xiàn)

1. bannister p, lord jm, comparative winter frost resistance of plant species from southern africa, australia, new zealand, and south america grown in a common environment (dunedin, new zealand). new zealand journal of botany, 2006. 44: 109-119.

2. pieruschka r, schurr u, jensen m, wolff wf, jahnke s, lateral diffusion of co₂ from shaded to illuminated leaf parts affects photosynthesis inside homobaric leaves. new phytologist, 2006. 169: 779-788.

3. shalygo nv, kozel nv, effect of bengal rose sensitizer on activity of the photosynthetic apparatus of green barley leaves journal of applied spectroscopy, 2006. 73(2): 301-304.

4. 肖月娥, 陳開(kāi)寧, 戴新賓, 陳小峰, 許曉明, 太湖中2種大型沉水被子植物適應(yīng)低光能力的比較. 植物生理學(xué)通訊, 2006. 42(3): 421-425.

5. bannister p, maegli t, dickinson kjm, halloy srp, knight a, lord jm, mark af, spencer kl, will loss of snow cover during climatic warming expose new zealand alpine plants to increased frost damage? . oecologia, 2005. 144(2): 245-256.

6. blouin m, zuily-fodil y, pham-thi a-t, laffray d, reversat g, pando a, tondoh j, lavelle p, belowground organism activities affect plant aboveground phenotype, inducing plant tolerance to parasites. ecology letters, 2005. 8(2): 202-208.

7. slapakauskas v, ruzgas v, chlorophyll fluorescence characteristics of different winter wheat varieties (triticum aestivum l.). agronomy research, 2005. 3(2): 203-209.

8. zapata jm, guéra a, esteban-carrasco a, martín m, sabater b, chloroplasts regulate leaf senescence: delayed senescence in transgenic ndhf-defective tobacco. cell death and differentiation 2005. 12: 1277-1284.

9. kasuga m, miura s, shinozaki k, yamaguchi-shinozaki k, a combination of the arabidopsis dreb1a gene and stress-inducible rd29a promoter improved drought- and low-temperature stress tolerance in tobacco by gene transfer plant cell and physiology, 2004. 45(3): 346-350.

10. morant-manceau a, pradier e, tremblin g, osmotic adjustment, gas exchanges and chlorophyll fluorescence of a hexaploid triticale and its parental species under salt stress journal of plant physiology, 2004. 161(1): 25-33.

11. kudoh s, imura s, kashino y, xanthophyll-cycle of ice algae on the sea ice bottom in saroma ko lagoon, hokkaido, japan. polar biology, 2003. 16: 86-97.

12. kudoh s, kashino y, imura s, ecological studies of aquatic moss pillars in antarctic lakes 3. light response and chilling and heat sensitivity of photosynthesis. polar bioscience, 2003. 16: 33-42.

13. motohashi r, ito t, kobayashi m, taji t, nagata n, asami t, yoshida s, yamaguchi-shinozak k, shinozaki k, functional analysis of the 37 kda inner envelope membrane polypeptide in chloroplast biogenesis using a ds-tagged arabidopsis pale-green mutant. the plant journal, 2003. 34(5): 719-731.

14. pshibytko nl, kalitukho ln, kabashnikova lf, effects of high temperature and water deficit on photosystem ii in hordeum vulgare leaves of various ages russian journal of plant physiology, 2003. 50(1): 44-51.

15. beer dd, larkum awd, photosynthesis and calcification in the calcifying algae halimeda discoidea studied with microsensors. plant cell and environment, 2001. 24(11): 1209-1217.

16. eckert m, kaldenhoff r, light-induced stomatal movement of se-lected arabidopsis thaliana mutants. journal of experimental botany, 2000. 51(349): 1435-1442.

17. shi l-x, lorkovi zj, oelmüller r, schröder wp, the low molecular mass psbw protein is involved in the stabilization of the dimeric photosystem ii complex in arabidopsis thaliana. the journal of biological chemistry, 2000. 275(48): 37945-37950.

18. christen g, stevens g, lukins pb, renger g, larkum awd, isolation and characterisation of oxygen evolving thylakoids from the marine prokaryote prochloron didemni febs letters, 1999. 449(2-3): 264-268.

19. jones rj, hoegh-guldberg o, larkum awd, schreiber u, temperature-induced bleaching of corals begins with impairment of the co₂ fixation mechanism in zooxanthellae. plant cell and environment, 1998. 21: 1219-1230.

20. salih a, hoegh-guldberg o, cox g. bleaching responses of symbiotic dinoflagellates in corals: the effects of light and elevated temperature on their morphology and physiology. in proceedings of the australian coral reef society 75th anniversary conference. 1997. heron island: the university of queensland, brisbane.

21. schreiber u, chlorophyll fluorescence and photosynthetic energy conversion: simple introductory experiments with the teaching-pam chlorophyll fluorometer. 1997, effeltrich, germany: heinz walz gmbh.