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產(chǎn)品名稱：上海人巨噬細(xì)胞移動抑制因子試劑盒,人巨噬細(xì)胞移動抑制因子試劑盒
聯(lián)系電話：021—60449724，13296089724
操作步驟：
參照說明書
靈敏度：
具體請查看說明書.
檢測范圍（RANGN）：
具體請查看說明書.
參考值：
產(chǎn)品用途:
科研ELISA檢測試劑盒.提供實(shí)驗(yàn)代測服務(wù).您只需將需要檢測的種屬(Human,Rat,Mouse,Rabbit,Monkey, Pig……)和檢測指標(biāo)(介素類、因子類)及標(biāo)本數(shù)量(48T/96T)通知公司業(yè)務(wù)員即可。在接到客戶標(biāo)本當(dāng)日起，現(xiàn)貨產(chǎn)品周內(nèi)將檢測報告交到客戶手中！
產(chǎn)品范圍:
人,小鼠,大鼠,豬,兔,其它動物細(xì)胞因子;細(xì)胞凋亡,活性多肽、自身抗體 ,血栓與止血,
骨代謝,肝纖維化,腫瘤,激素內(nèi)分泌、自身抗體科研ELISA檢測試劑盒

試劑的準(zhǔn)備：

1.   標(biāo)準(zhǔn)品：標(biāo)準(zhǔn)品的系列稀釋應(yīng)在實(shí)驗(yàn)時準(zhǔn)備，不能儲存。稀釋將標(biāo)準(zhǔn)品振蕩混勻。
2.   洗滌緩沖液（50×）的稀釋：蒸餾水50倍稀釋。

試劑盒性能：

 1.   靈敏度：小的檢測濃度小于1號標(biāo)準(zhǔn)品。稀釋度的線性。樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.990。

2.   特異性：不與其它細(xì)胞因子反應(yīng)。

3.   重復(fù)性：板內(nèi)、板間變異系數(shù)均小于10%。

Calculate
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer ａnd each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
Storage ａnd validity
1．Storage：  2-8℃.
2．validity： six months.