這種競爭性ELISA試劑盒用于測定細胞中游離和葡萄糖醛酸化的20-HETE水平提取物和細胞培養(yǎng)基。該試劑盒類似于我們的20-HETE ELISA試劑盒（Cat#20H1），但也包含β-葡萄糖醛酸酶。每個96孔板足以進行多達12個游離樣品的一式三份分析和12個葡萄糖醛酸化樣品。正常大鼠腎臟（NRK）細胞的花生四烯酸（AA）依賴性20-HETE形成活性100人中20-HETE形成的ELISA測量mL培養(yǎng)基（總體積的1/10）和隨后的細胞裂解物培養(yǎng)5 x 104，在含有或不含有100µM AA和1mM NADPH的情況下將細胞培養(yǎng)30分鐘。

Detroit R&D 20-HETE/葡萄糖醛酸酶ELISA試劑盒相關(guān)研究： 

用于一步純化的20-HETE免疫親和柱 20H33

生物靶標(biāo) 20-HETE ELISA 試劑盒 20H1

Detroit R&D 20-HETE/葡萄糖醛酸酶ELISA試劑盒部分參考文獻：

1. Liu et al. Association of a functional cytochrome P450 4F2 haplotype with urinary 20-HETE ａnd hypertension. J. Am. Soc. Nephrol. 19, 714-721, 2008.

2. Meseguer et al. Kidney androgen-regulated protein transgenic mice show hypertension ａnd renal alterations mediated by oxidative stress.Circulation. 119, 1908-1917, 2009.

3. Dolegowska, B., Blogowski, W., Domanski, L.. Is it possible to predict the early post-transplant allograft function using 20-HETE measurements? A preliminary report. Transpl Int. 2009 22:546-553

4. Liu et al. Overexpression of cytochrome P450 4F2 in mice increases 20-hydroxyeicosatetraenoic acid production ａnd arterial blood pressure. Kidney. International. 75, 1288-1296, 2009.

5. Wang et al. Selective inhibitors of CYP2J2 related to terfenadine exhibit activity strongly against human cancers in vitro ａnd in vivo. J.Pharmacol. Exp. Ther. 329, 908-918, 2009.

6. Tunctan et al. A synthetic analogue of 20-HETE, 5,14-HEDGE, reverses endotoxin-Induced hypotension via increased 20-HETE levels associated with decreased iNOS protein expression ａnd vasodilator prostanoid production in rats. Basic Clin. Pharmacol. Toxicol. 106, 378-388, 2010.

Detroit R&D注于心血管疾病、癌癥的研究，診斷和治療域。涉及環(huán)境健康科學(xué)、高血壓、乳腺癌和列腺癌相關(guān)研究域。艾美捷科技是Detroit R&D的中國代理商，為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務(wù)。

來源：https://www.amyjet.com/news/Detroit-R-D-new.shtml