Background: The EndoAlert™ Endotoxin Plate Kit is a kinetic, colorimetric assay for the quantitative determination of bacterial Endotoxin in aqueous solutions. Endotoxin, a bacterial lipopolysaccharide, is one of the major cell wall components of most gram-negative bacteria. The EndoAlert™ Endotoxin Plate Kit detects low levels of Endotoxin ａnd is therefore a useful tool to assess the integrity of biological ａnd environmental samples. The detection ranges from 0.01 - 10 Endotoxin units (EU/mL). The EndoAlert™ Endotoxin Plate Kit is a quantitative version of the reaction first described by Levin ａnd Bang in 1968. The test is based upon an enzymatic cascade where Endotoxin activates Factor C in Limulus Amebocyte Lysate (LAL) which in turn activates Factor B. Factor B activates Proclotting Enzyme which then activates Clotting Enzyme. A colorless synthetic peptide substrate is hydrolyzed by Clotting Enzyme to generate a yellow color which can be measured by a spectrophotometer at 405 nm. The degree of color resulting from the reaction is proportional to the amount of Endotoxin in the test sample ａnd can be calculated using a standard curve.

Label: Unconjugated

Expiration: See kit ｉnsert for complete instructions

Storage Condition:Store all kit reagents at 2-8 °C in the dark. Avoid storing kits for extended periods (more than 24 hours) at room temperature. See kit protocol for complete details.

Synonyms: Endotoxin Test Kit, Endotoxin level, Endotoxin Detection, bacterial Endotoxin, Limulus Amebocyte Lysate (LAL)

Assay Dilutions: All assays should be optimized by the user. Recommended dilutions (if any) may be listed below

艾美捷內(nèi)毒素檢測試劑盒中文說明：

艾美捷內(nèi)毒素檢測試劑盒是一種用于定量測定水溶液中細菌內(nèi)毒素的動力學比色測定法。內(nèi)毒素是一種細菌脂多糖，是大多數(shù)革蘭氏陰性細菌的主要細胞壁成分。該檢測低水平的內(nèi)毒素，因此是評估生物和環(huán)境樣品完整性的有用工具。檢測范圍為0.01-10內(nèi)毒素單位（EU/mL）。

艾美捷內(nèi)毒素檢測試劑盒基本參數(shù)：

中文名稱：EndoAlert™ 內(nèi)毒素檢測試劑盒

英文名字：EndoAlert™ Endotoxin Plate Kit - KMA-0100

編號：KMA-0100

規(guī)格：1Kit

檢測范圍：0.01-10內(nèi)毒素單位（EU/mL）。孵育60分鐘。

將所有試劑盒試劑儲存在2-8°C的黑暗中。避免在室溫下長時間（超過24小時）存放試劑盒。

艾美捷內(nèi)毒素檢測試劑盒原理：

該是Levin和Bang于1968年shou次描述的反應的定量版本。該測試基于酶聯(lián)反應，其中內(nèi)毒素激活鱟血細胞裂解物（LAL）中的因子C，進而激活因子B。因子B激活Proclotting酶，然后激活凝血酶。無色合成肽底物通過凝集酶水解以產(chǎn)生黃色，該黃色可通過分光光度計在405nm下測量。反應產(chǎn)生的顏色程度與測試樣品中內(nèi)毒素的量成比例，并且可以使用標準曲線計算。

來源：https://www.amyjet.com/products/KMA-0100.shtml