圖：PROTEOSTAT®染料的聚集溫度不等于使用SYPRO®橙色獲得的熔化溫度。

SYPRO橙色染料是一種環(huán)境敏感染料，與未折疊蛋白質(zhì)的疏水區(qū)域結合�？捎糜跍y量Tm。Tm是熱展開曲線的溫度中點。在溫度中點Tm，樣品中一半的靶蛋白被展開，樣品中的一半靶蛋白保持折疊。展開不依賴于相鄰的蛋白質(zhì)。ProteoStat是一種分子轉(zhuǎn)子染料，與蛋白質(zhì)聚集體表面結合。標簽是指熱聚集曲線的中點。蛋白質(zhì)展開，然后聚集。聚集依賴于鄰近的蛋白質(zhì)。蛋白質(zhì)濃度越高，聚集的趨勢就越大。

艾美捷熱轉(zhuǎn)移穩(wěn)定性檢測試劑盒基本參數(shù)：

中文名稱：熱轉(zhuǎn)移穩(wěn)定性檢測

英文名字：PROTEOSTAT® Thermal shift stability assay kit

編號：ENZ-51027-K400

規(guī)格：400tests

使用/穩(wěn)定性：正確儲存后，試劑盒組件在產(chǎn)品標簽上注明的日期之是穩(wěn)定的。將套件儲存在-20˚C的無霜冷凍箱中，或-80˚C下長期儲存。

艾美捷熱轉(zhuǎn)移穩(wěn)定性檢測試劑盒部分文獻參考：

Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system: S. Murakami, et al.; Sci. Rep. 9, 671 (2019), Application(s): Purified IgG or Trastuzumab, Abstract; Full Text

Structural insights into humanization of anti-tissue factor antibody 10H10: A. Teplyakov, et al.; MAbs. 10, 269 (2018), Application(s): Monoclonal antibodies, Abstract; Full Text

Protein catalyzed capture agents with tailored performance for in vitro ａnd in vivo applications: M.B. Coppock, et al.; Biopolymers 108, e22934 (2017), Application(s): Stability of peptide-based ligands, Abstract;

Time-Dependent Protein Thermostability Assay: I. Vandecaetsbeek, et al.; Methods Mol. Biol. 1377, 79 (2016), Abstract;

Effect of Polyethylene Glycol Conjugation on Conformational ａnd Colloidal Stability of a Monoclonal Antibody Antigen-Binding Fragment (Fab＇): C. Roque, et al.; Mol. Pharm. 12, 562 (2015), Abstract;

來源：https://www.amyjet.com/products/ENZ-51027-K400.shtml