Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion ａnd two antigen binding Fab portions joined together by disulfide bonds ａnd therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures ａnd is the most cost effective.

Usage

Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.

Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6

Preservative: None

Suggested Working Concentration or Dilution Range:

10-20 µg / ml

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

基于免疫電泳和/或ELISA抗體，抗體與人IgA的重鏈反應(yīng)，但不與人IgG或IgM反應(yīng)。未檢測到針對非免疫球蛋白血清蛋白的抗體。該抗體可能與其他物種的IgA發(fā)生交叉反應(yīng)。

通過免疫親和色譜法從抗血清中分離出完整的 IgG 抗體作為完整分子。它們有一個(gè)Fc部分和兩個(gè)抗原結(jié)合Fab部分通過二硫鍵連接在一起，因此它們是二價(jià)的。據(jù)報(bào)道，平均分子量約為160kDa。整個(gè)IgG形式的抗體適用于大多數(shù)免疫檢測程序，并且非常具有成本效益。

艾美捷AffiniPure兔抗人血清伊加，α鏈特異性#309-005-011用法：

物理狀態(tài)：無菌過濾液

存儲： 在無菌條件下儲存在2-8°C。使用當(dāng)天準(zhǔn)備工作稀釋液。

有效期：自收到之日起一年。如果測試結(jié)果可以接受預(yù)期用途，則有效期可以延長。

純度：使用與瓊脂糖珠偶聯(lián)的抗原通過免疫親和色譜法從抗血清中純化抗體。

緩沖液：0.01M磷酸鈉，0.25M氯化鈉，pH 7.6

防腐劑：無

建議工作濃度或稀釋范圍：

10-20μg/ ml

稀釋因子以范圍的形式呈現(xiàn)，因?yàn)榧严♂尪仁窃S多因素的函數(shù)，例如抗原密度，滲透性等。實(shí)際使用的稀釋度必須根據(jù)經(jīng)驗(yàn)確定。

AffiniPure兔抗人血清伊加，α鏈特異性#309-005-011

目標(biāo)： 人類

宿主：兔

抗體形式：全 IgG 特異性：血清 IgA，α鏈特異性

偶聯(lián)物：非偶聯(lián)

產(chǎn)品類別：全 IgG

親和純化抗體

克隆性：多克隆

RRID：AB_2339627

來源;https://www.amyjet.com/products/309-005-011.shtml