何為i-MICST技術(shù)？

i-MICST技術(shù)（integrated Magnetic Immuno-Cell Sorting ａnd Transfection/ Transduction，集成磁性免疫細(xì)胞分選和轉(zhuǎn)染/轉(zhuǎn)導(dǎo)）是一種新平臺，可以在磁性細(xì)胞純化柱上直接對細(xì)胞進(jìn)行基因修飾。該技術(shù)將細(xì)胞分離和基因修飾結(jié)合在一個簡單、高效和可靠的集成系統(tǒng)中。

Viro-MICST Transduction Reagent allows genetically modifying cells directly on magnetic cell purification columns. Based on the i-MICST™ Technology (Integrated Magnetic Immuno-Cell Sorting ａnd Transfection/Transduction), this technology combines cell isolation ａnd genetic modification in one simple ａnd reliable integrated system. For efficient ａnd selective gene delivery to target cells, Viro-MICST can be used in combination with any viral vector ａnd allows you to reduce cell manipulation steps ａnd save time ａnd material.

Highly efficient

Rapid, simple ａnd ready to use

Suitable for all cells

Sizes:

250 µL: suitable for 25 to 50 assays for 106 cells ａnd MOI=1

500 µL: suitable for 50 to 100 assays for 106 cells ａnd MOI=1

1 mL: suitable for 100 to 200 assays for 106 cells ａnd MOI=1

為什么使用Viro-MICST試劑

與常規(guī)轉(zhuǎn)導(dǎo)方法相比，艾美捷Viro-MICST試劑可提高低滴度病毒制劑的轉(zhuǎn)導(dǎo)效率，并可幫助您減少細(xì)胞操作步驟，節(jié)省時間和載體材料。

在一個可靠的集成系統(tǒng)中分離和轉(zhuǎn)導(dǎo)細(xì)胞；

在低MOI下，有高轉(zhuǎn)導(dǎo)效率；

轉(zhuǎn)導(dǎo)過程的加速和吸附的同步化；

適用于敏感細(xì)胞類型，如原代細(xì)胞和干細(xì)胞。

Viro-MICST的轉(zhuǎn)導(dǎo)。用CD45、CD2或CD15微珠標(biāo)記人PBMC。然后將每種條件加載到i）一個未修飾的MACS®LS柱中，然后使用標(biāo)準(zhǔn)慢病毒（標(biāo)準(zhǔn)感染）感染選定的細(xì)胞，或ii）一個無修飾的MACS®LS柱，然后用Viro-MICST/LV.eGFP復(fù)合物修飾的MAC斯®LS柱。通過流式細(xì)胞術(shù)測量感染效率。

Viro-MICST試劑試劑相關(guān)研究：

#VMX050 VIRO-MICST TRANSDUCTION REAGENT SAMPLE 轉(zhuǎn)導(dǎo)和感染增強(qiáng)劑 所有病毒載體

#VMX250 VIRO-MICST 250 TRANSDUCTION REAGENT 轉(zhuǎn)導(dǎo)和感染增強(qiáng)劑 所有病毒載體

#VMX500 VIRO-MICST 500 TRANSDUCTION REAGENT 轉(zhuǎn)導(dǎo)和感染增強(qiáng)劑 所有病毒載體

#VMX1000 VIRO-MICST 1000 TRANSDUCTION REAGENT 轉(zhuǎn)導(dǎo)和感染增強(qiáng)劑 所有病毒載體

#VMXS100 ViroMICST Stem 100 transductions 轉(zhuǎn)導(dǎo)和感染增強(qiáng)劑 干細(xì)胞

#VMXS300 ViroMICST Stem 300 transductions 轉(zhuǎn)導(dǎo)和感染增強(qiáng)劑 干細(xì)胞

來源：https://www.amyjet.com/brand/Viro-MICST.shtml