The plant chromatin immunoprecipitation (ChIP) kit provides all the reagents needed for the chromatin immunoprecipitation reaction of plant cell samples. In addition, there is also a ChIP grade dimethyl histone H3-K9 antibody ａnd a negative control antibody (IgG of normal mice) in the kit. The chromatin extracted from the cell samples is properly interrupted, Then it is added to the micropores to undergo an immune reaction with the antibodies adsorbed on their surface. The DNA specifically bound to the micropores is released from the antibody capture protein DNA complex, flipped over, ａnd purified through our company＇s specially designed high-speed centrifuge column. The eluted DNA can be used for various subsequent analyses

艾美捷植物染色質(zhì)免疫沉淀(ChIP)試劑盒#P-2014-24可以有效進(jìn)行體外檢測(cè)植物中蛋白質(zhì)-DNA相互作用，操作方便.操作過(guò)程不到六小時(shí)就可以獲得比其它試劑盒更好的結(jié)果.試劑盒獲得的染色質(zhì)可用來(lái)進(jìn)行定性和定量PCR、southern 印跡以及DNA微陣列分析.

植物染色質(zhì)免疫沉淀(ChIP)試劑盒提供了對(duì)植物細(xì)胞樣品進(jìn)行染色質(zhì)免疫沉淀反應(yīng)所需的所有試劑.并且，試劑盒中還有一種ChIP二甲基組蛋白H3-K9抗體和一種陰性對(duì)照抗體（正常小鼠的IgG）.從細(xì)胞樣品中提取出來(lái)的染色質(zhì)進(jìn)行適當(dāng)?shù)拇驍�，然后加入到微孔中與其表面上吸附的抗體發(fā)生免疫反應(yīng).特異性結(jié)合到微孔上的DNA從抗體-捕獲蛋白-DNA復(fù)合物上釋放出來(lái)，翻轉(zhuǎn)后，通過(guò)本公司門(mén)設(shè)計(jì)的高速離心柱純化.洗脫下來(lái)的DNA可用于隨后的各種分析.

該植物染色質(zhì)免疫沉淀(ChIP)試劑盒組成：

Components 24 readions     48 regdions  

P-2014-24       P-2014-48  

CP1 (Wash Buffer) 28ml        2x28ml  

CP2  (Antibody Buffer)     15ml   30ml

CP3C (5X Lysis Buffer )      12ml    24ml

CP3D (Lysis Buffer II) 3ml   6ml  

CP3E (Lysis Buffer II) 2ml   4ml  

CP3F (Lysis Buffer M) 1.5 ml  5ml  

CP4 (ChP  Dilution Buffer) 2ml  6ml  

CP5 (DNA Release Buffer) 2ml  2x2ml  

CP6( Reverse Buffer) 2ml   2x2ml  

CP7(Binding  Buffer) 5ml  8  ml  

CP8 (Elution Buffer) 0.6ml   1.2ml  

Protease Inhibitor Cocktail (100X)* 25μl  50μl  

Non-immune  lgG  (1mg/m)* 10μ 10μ  

Anti-Dimethyl  H3-K9 (1 mg/ml)* * 5μl   8μl  

Proteinase K (10 mg/ml)* 25μl    50μl  

8-Well Assay Strips (with frame) 3  6

8-Well Srip Caps 3 6

F-Spin  Column 30  50  

F-Collection Tube 30   50  

*Spin the solution down to the boffom before use.  

該植物染色質(zhì)免疫沉淀(ChIP)試劑盒特點(diǎn)：

1、市場(chǎng)上同類產(chǎn)品中較為快捷的試劑盒，整個(gè)處理過(guò)程不到6小時(shí)；

2、96孔板模式使研究人員能根據(jù)自己需要選擇手工或是高通量分析；

3、附有DNA純化柱子：大量減少不必要的重復(fù)勞動(dòng)；

4、操作簡(jiǎn)便、結(jié)果可靠、統(tǒng)一的分析條件；

來(lái)源：https://www.amyjet.com/products/P-2014-24.shtml