Magnetic nanoparticles are made of completely biodegradable iron oxide ａnd coated with specific proprietary cationic molecules, with different applications. Their binding to gene vectors (DNA, siRNA, ODN, viruses, etc.) is achieved through salt induced colloidal aggregation ａnd electrostatic interactions. Then, under the influence of an external magnetic field generated by a specific magnetic plate, magnetic particles are concentrated on the cell. The uptake of genetic material by cells is accomplished through two natural biological processes, endocytosis ａnd pinocytosis. Therefore, compared to other physical transfection methods that destroy, generate pores, or shock cell membranes, the membrane structure remains intact. Then, according to the formula used, nucleic acids are released into the cylasm through different mechanisms. The first is the proton sponge effect caused by cationic polymers coated on nanoparticles, which promotes the osmotic expansion of the endosomes, damages the membrane of the endosomes, ａnd releases DNA within cells. The second is the destruction of cationic lipids enveloping the endosomes on particles, which release nucleic acids into the cell through the flipping ａnd charge neutralization of negative lipids in the cell. The third is the common virus mechanisms during virus use.

艾美捷Mega Magnetic Plate超大磁性板（MF14000）可同時(shí)容納4個(gè)培養(yǎng)皿或培養(yǎng)皿。

OZ Biosciences磁性板適用于所有磁感染試劑和所有細(xì)胞培養(yǎng)皿（384、96、4、24、12、6孔板、35、60、90和100 mm培養(yǎng)皿、T-25、T-75和任何其他燒瓶）可以在孵化器和機(jī)器人中使用刻在磁性板上的OZ生物科學(xué)指示要執(zhí)行的一側(cè)磁性感染。筆記。磁轉(zhuǎn)染™ 技術(shù)需要適當(dāng)?shù)拇艌?chǎng)來(lái)磁化納米顆粒在溶液中，形成一個(gè)非常強(qiáng)的梯度來(lái)吸引納米顆粒，并覆蓋所有板的表面。

該磁性轉(zhuǎn)染（Magnetofection）原理是將核酸、轉(zhuǎn)染試劑或病毒與特異性陽(yáng)離子磁性納米顆粒結(jié)合。然后濃縮所得的分子復(fù)合物并轉(zhuǎn)運(yùn)到由適當(dāng)磁場(chǎng)支持的細(xì)胞中。通過(guò)這種方式，利用施加在基因載體上的磁力，可以非�？焖俚貙⒄麄�(gè)載體劑量集中吸引到細(xì)胞上，使100%的細(xì)胞接觸到顯著的載體劑量，并促進(jìn)細(xì)胞攝取。

磁性納米顆粒由完全可生物降解的氧化鐵制成，涂有特定的有陽(yáng)離子分子，應(yīng)用不同。它們與基因載體（DNA、siRNA、ODN、病毒等）的結(jié)合是通過(guò)鹽誘導(dǎo)的膠體聚集和靜電相互作用實(shí)現(xiàn)的。然后，通過(guò)由特定磁性板產(chǎn)生的外部磁場(chǎng)的影響，磁性顆粒集中在細(xì)胞上。細(xì)胞對(duì)遺傳物質(zhì)的攝取是通過(guò)內(nèi)吞作用和胞飲作用這兩個(gè)自然的生物過(guò)程完成的。 因此，與破壞、產(chǎn)生孔或電擊細(xì)胞膜的其它物理轉(zhuǎn)染方法相比，膜的結(jié)構(gòu)保持完整。然后，根據(jù)所使用的配方，通過(guò)不同的機(jī)制將核酸釋放到細(xì)胞質(zhì)中。先是由包被在納米顆粒上的陽(yáng)離子聚合物引起的質(zhì)子海綿效應(yīng)，其促進(jìn)核內(nèi)體滲透膨脹、破壞核內(nèi)體膜和細(xì)胞內(nèi)DNA釋放。 第二是核內(nèi)體被包被在顆粒上的陽(yáng)離子脂質(zhì)破壞，這些顆粒通過(guò)細(xì)胞負(fù)性脂質(zhì)的翻轉(zhuǎn)和電荷中和將核酸釋放到細(xì)胞中。 第三是病毒使用時(shí)常見(jiàn)的病毒機(jī)制。

來(lái)源：https://www.amyjet.com/products/OZB-MF10096.shtml