Benefits:

Highly efficient: achieve RNAs delivery in all cells

Ready-to-use: no need for additional buffer

Low nucleic acid amount : minimized toxicity

Protects RNAs against degradation

Compatible with any culture medium

Serum compatible : Medium changed not required

mRNA transfection provides several advantages over plasmid DNA delivery :

No need for nuclear uptake. mRNA translation into proteins occurs in the cylasm.

Faster protein expression than DNA transfection

No genomic integration

Protein expression in a total promoter-independent manner

Additional products for CRISPR Cas9 experiments:

PolyMag CRISPR for Genome editing using expression plasmids

ProDeliverIN CRISPR for Cas9 protein delivery

ViroMag CRISPR to enhance transduction efficiency of CRISPR/Cas9 viruses

基于TEE技術(shù)的艾美捷 RmesFect™ CRISPR轉(zhuǎn)染試劑為mRNA/gRNA轉(zhuǎn)染而設(shè)計(jì)，具有高效率和低毒性的特點(diǎn)。RmesFect™在多種細(xì)胞中都是有效的。

OZ Biosciences RmesFect CRISPR轉(zhuǎn)染試劑特點(diǎn)：

高效：在所有細(xì)胞中實(shí)現(xiàn)RNA遞送

即用型：無需額外的緩沖液

低核酸量：毒性小化

保護(hù) RNA 免受降解

與任何培養(yǎng)基兼容

血清兼容：無需更換培養(yǎng)基

圖 1：根據(jù)標(biāo)準(zhǔn)方案，使用 RmesFect CRISPR 轉(zhuǎn)染編碼 GFP 和 RFP（各 24.0μg）的 mRNA 轉(zhuǎn)染 DC25 細(xì)胞。轉(zhuǎn)染后24h在顯微鏡熒光下分析圖像。

與質(zhì)粒DNA遞送相比，mRNA轉(zhuǎn)染具有以下優(yōu)勢：

無需核吸收。mRNA翻譯成蛋白質(zhì)發(fā)生在細(xì)胞質(zhì)中。

蛋白表達(dá)速度快于DNA轉(zhuǎn)染

無基因組整合

以完全啟動(dòng)子非依賴的方式表達(dá)蛋白質(zhì)

用于CRISPR Cas9實(shí)驗(yàn)的其他產(chǎn)品：

PolyMag CRISPR用于使用表達(dá)質(zhì)粒進(jìn)行基因組編輯

ProDeliverIN CRISPR用于 Cas9 蛋白遞送

ViroMag CRISPR提高CRISPR/Cas9病毒的轉(zhuǎn)導(dǎo)效率

來源：https://www.amyjet.com/products/OZB-RMC70500.shtml