Isotype Mouse IgG2a, κ

Recommended Isotype Control(s) InVivoMAb mouse IgG2a isotype control, unknown specificity

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Immunogen Mouse spleen ａnd bone marrow cells enriched for NK1+ cells

Reported Applications in vivo NK cell depletion

Flow cytometry

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from tissue culture supernatant in an animal free facility

Purification Protein A

RRID AB_1107737

Molecular Weight 150 kDa

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

PK136單克隆抗體與小鼠NK1.1反應(yīng)，也稱為CD161b / CD161c，KLRB1，NKR-P1A和Ly-55。NK1.1是一種具有C型凝集素結(jié)構(gòu)域的II型整合膜糖蛋白，由Klrb1c/NKR-P1C基因編碼。NK1.1在NK細(xì)胞活化和分化，IFN-γ產(chǎn)生，細(xì)胞毒性顆粒釋放中起作用，并被認(rèn)為參與Th2細(xì)胞的產(chǎn)生。NK1.1主要在NK細(xì)胞上表達(dá)為二硫鍵連接的同源二聚體，然而，它也在NK-T細(xì)胞上表達(dá)，NK-T細(xì)胞是一種罕見的T淋巴細(xì)胞群。NK 1.1僅由小鼠的C57BL / 6，F(xiàn)VB / N和NZB菌株表達(dá)，而不是AKR，BALB / c，CBA / J，C3H，DBA / 1，DBA / 2，NOD，SJL和129株表達(dá)。

艾美捷InVivoMAb抗小鼠NK1.1#BE0036基本參數(shù)：

同種型 小鼠IgG2a， κ

推薦的同種型對(duì)照 體內(nèi)MAb小鼠IgG2a同種型對(duì)照，特異性未知

推薦的稀釋緩沖液 體內(nèi)純 pH 7.0 稀釋緩沖液

免疫原 富含NK1+細(xì)胞的小鼠脾臟和骨髓細(xì)胞

報(bào)告的應(yīng)用程序 體內(nèi)NK細(xì)胞去除

流式細(xì)胞術(shù)

配方 PBS，pH 7.0

不含穩(wěn)定劑或防腐劑

內(nèi)毒素 <2EU/mg （<0.002EU/μg）

通過LAL凝膠凝血測(cè)定法測(cè)定

純度 >95%

由 SDS-PAGE 決定

無 菌 0.2 μM 過濾

生產(chǎn) 從無動(dòng)物設(shè)施中的組織培養(yǎng)上清液中純化

純化 蛋白A

里德 AB_1107737

分子量 150 千達(dá)

存儲(chǔ) 抗體溶液應(yīng)以4°C的儲(chǔ)備濃度儲(chǔ)存。 不要凍結(jié)。

InVivoMAb抗小鼠NK1.1部分文獻(xiàn)引用：

in vivo NK cell depletion

Ghasemi, R., et al. (2016). "Selective targeting of IL-2 to NKG2D bearing cells for improved immunotherapy" Nat Commun 7: 12878.

in vivo NK cell depletion, Flow Cytometry

Maltez, V. I., et al. (2015). "Inflammasomes Coordinate Pyroptosis ａnd Natural Killer Cell Cytotoxicity to Clear Infection by a Ubiquitous Environmental Bacterium" Immunity 43(5): 987-997.

in vivo NK cell depletion, Flow Cytometry

Wensveen, F. M., et al. (2015). "NK cells link obesity-induced adipose stress to inflammation ａnd insulin resistance" Nat Immunol 16(4): 376-385.

in vivo NK cell depletion

Yamada, D. H., et al. (2015). "Suppression of Fcgamma-receptor-mediated antibody effector function during persistent viral infection" Immunity 42(2): 379-390.

來源：https://www.amyjet.com/products/BXC-BE0036-1mg.shtml