The RMP1-14-CP162 monoclonal antibody is a chimeric version of the original RMP1-14 antibody. The variable domain sequences are identical to the original RMP1-14 but the constant region sequences have been switched from rat IgG2a to mouse IgG1. The RMP1-14-CP162 antibody contains no Fc mutations just as the original rat IgG2a antibody does not. RMP1-14-CP162 reacts with mouse PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 ａnd CD8 thymocytes as well as activated T ａnd B lymphocytes ａnd myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1’s structure includes a ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 ａnd PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, ａnd T-cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance ａnd prevention of autoimmune disease in mice as PD-1 knockout animals show dilated cardiomyopathy, splenomegaly, ａnd loss of peripheral tolerance. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, ａnd breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 ａnd its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being explored as cancer treatments.

BioXCell RecombiMAb小鼠PD-1(CD279)抗體：

RMP1-14-CP162單克隆抗體是原始RMP1-14抗體的嵌合版本�？勺兘Y(jié)構(gòu)域序列與原始RMP1-14相同，但恒定區(qū)域序列已從大鼠IgG2a切換到小鼠IgG1。RMP1-14-CP162抗體不含F(xiàn)c突變，就像原始大鼠IgG2a抗體不含一樣。 RMP1-14-CP162與小鼠PD-1（程序性死亡-1）反應(yīng)，也稱為CD279。PD-1是由Pdcd50基因編碼的55-1 kDa細(xì)胞表面受體，屬于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺細(xì)胞以及活化的T和B淋巴細(xì)胞和骨髓細(xì)胞上短暫表達(dá)。PD-1在成功消除抗原后表達(dá)下降。

此外，Pdcd1 mRNA在B細(xì)胞階段的發(fā)育B淋巴細(xì)胞中表達(dá)。PD-1的結(jié)構(gòu)包括ITIM（基于免疫受體酪氨酸的抑制基序），表明PD-1負(fù)調(diào)節(jié)TCR信號。PD-1通過結(jié)合其兩個(gè)配體PD-L1和PD-L2來發(fā)出信號，PD-L7和PD-L1都是B1家族的成員。配體結(jié)合后，PD-1信號傳導(dǎo)抑制T細(xì)胞活化，導(dǎo)致增殖減少，細(xì)胞因子產(chǎn)生和T細(xì)胞死亡。此外，已知PD-1在小鼠外周耐受和預(yù)防自身免疫性疾病中起關(guān)鍵作用，因?yàn)镻D-1基因敲除動(dòng)物表現(xiàn)出擴(kuò)張型心肌病，脾腫大和外周耐受性喪失。誘導(dǎo)PD-L8表達(dá)在許多腫瘤中很常見，包括鱗狀細(xì)胞癌、結(jié)腸腺癌和乳腺腺癌。

BioXCell艾美捷RecombiMAb小鼠PD-1(CD279)抗體規(guī)格：

#  BXC-CP162

Isotype Mouse IgG1, κ

Recommended Isotype Control(s) InVivoPlus mouse IgG1 isotype control, unknown specificity

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Immunogen Syrian Hamster BKH cells transfected with mouse PD-1 cDNA

Reported Applications in vivo blocking of PD-1/PD-L signaling*

*Reported for the original rat IgG2a RMP1-14 antibody

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <1EU/mg (<0.001EU/μg)

Determined by LAL gel clotting assay

Aggregation <5%

Determined by DLS

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from CHO cell supernatant in an animal free facility

Purification Protein A

RRID AB_2927529

Molecular Weight 150 kDa

Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

同種型 小鼠IgG1， κ

推薦的同種型對照 體內(nèi)加小鼠IgG1同種型對照，特異性未知

推薦的稀釋緩沖液 體內(nèi)純 pH 7.0 稀釋緩沖液

免疫原 用小鼠PD-1 cDNA轉(zhuǎn)染的敘利亞倉鼠BKH細(xì)胞

報(bào)告的應(yīng)用程序 體內(nèi)阻斷PD-1/PD-L信號傳導(dǎo)**

報(bào)道了原始大鼠IgG2a RMP1-14抗體

配方 PBS，pH 7.0

不含穩(wěn)定劑或防腐劑

內(nèi)毒素 <1EU/mg （<0.001EU/μg）

通過LAL凝膠凝血測定法測定

集合體 <5%

由 DLS 確定

純度 >95%

由 SDS-PAGE 決定

無 菌 0.2 μM 過濾

生產(chǎn) 從無動(dòng)物設(shè)施中的CHO細(xì)胞上清液中純化

純化 蛋白A

里德 AB_2927529

分子量 150 千達(dá)

鼠病原體檢測 外切粒菌/鼠痘病毒： 陰性 漢坦病毒： 陰性 K 病毒： 陰性乳酸脫氫酶升高病毒： 陰性 淋巴細(xì)胞性脈絡(luò)叢腦膜炎病毒： 陰性小鼠腺病毒： 陰性小鼠巨細(xì)胞病毒： 陰性小鼠肝炎病毒： 陰性小鼠分鐘病毒： 陰性小鼠諾如病毒： 陰性小鼠細(xì)小病毒： 陰性

小鼠輪狀病毒：肺支原體陰性：陰性 小鼠肺炎病毒：陰性 多瘤病毒：陰性呼腸孤病毒篩查：陰性 仙臺病毒：陰性 泰勒鼠腦脊髓炎：陰性

存儲 抗體溶液應(yīng)以4°C的儲備濃度儲存。 不要凍結(jié)。

來源：https://www.amyjet.com/products/BXC-CP162-1mg.shtml