艾美捷一氧化氮(總)檢測試劑盒基于硝酸鹽還原酶將硝酸鹽酶轉(zhuǎn)化為亞硝酸鹽，然后通過Griess反應(yīng)形成有色偶氮染料產(chǎn)物。通過測定540 - 570 nm處的吸光度進(jìn)行定量。每孔僅需0.050ml稀釋樣品，節(jié)省了寶貴的樣品。靈敏、高效、簡便的總一氧化氮檢測方法，只需40應(yīng)用。

一氧化氮(總)檢測試劑盒基參：

敏感性： 0.625 微摩爾/升（范圍 3.125 - 100 微摩爾/升）

應(yīng)用： 比色檢測

應(yīng)用說明： 用于定量測定培養(yǎng)物上清液、血漿、血清、唾液和尿液中任何物種的總一氧化氮。引用的樣本類型包括組織（參考文獻(xiàn)）。

物種反應(yīng)性： 物種獨立

使用/穩(wěn)定性： 將所有組分儲存在 4°C，硝酸鹽還原酶除外，在 -20°C 下儲存。

航運： 在藍(lán)冰上運輸

內(nèi)容： 微量滴定板，硝酸鹽還原酶，硝酸鹽還原酶儲存緩沖液，NADH，硝酸鹽標(biāo)準(zhǔn)品，反應(yīng)緩沖液濃縮物，格里斯試劑I，格里斯試劑II

一氧化氮(總)檢測試劑盒（ADI-917-020）特點：

Efficient - only 50 µl diluted sample needed, saving your precious sample

Sensitive - detect as little as 0.625 µmol/L

Trusted - widely cited in peer-reviewed publications

Fast - results from up to 80 samples in duplicate in just 40 minutes

一氧化氮(總)檢測試劑盒部分文獻(xiàn)參考：

1-Oleyl-lysophosphatidic acid (LPA) promotes polarization of BV-2 ａnd primary murine microglia towards an M1-like phenotype: I. Plastira, et al.; J. Neuroinflammation 13, 205 (2016), Application(s): iNOS activity assessment (through measuring accumulated total nitrate levels) primary microglia ａnd BV-2 cell supernatant, Abstract;

Neuronal NOS Activates Spinal NADPH Oxidase 2 Contributing to Central Sigma-1 Receptor-Induced Pain Hypersensitivity in Mice: S.R. Choi, et al.; Biol. Pharm. Bull. 39, 1922 (2016);

Peroxiredoxin 6 expression is inversely correlated with nuclear factor-κB activation during Clonorchis sinensis infestation: J.H. Pak, et al.; Free Radic. Biol. Med. 99, 273 (2016), Application(s): Measuring nitrite/nitrate concentration in culture medium;

來源：https://www.amyjet.com/products/ADI-917-020.shtml