艾美捷一氧化氮(總)檢測(cè)試劑盒基于硝酸鹽還原酶將硝酸鹽酶轉(zhuǎn)化為亞硝酸鹽，然后通過(guò)Griess反應(yīng)形成有色偶氮染料產(chǎn)物。通過(guò)測(cè)定540 - 570 nm處的吸光度進(jìn)行定量。每孔僅需0.050ml稀釋樣品，節(jié)省了寶貴的樣品。靈敏、高效、簡(jiǎn)便的總一氧化氮檢測(cè)方法，只需40分鐘

應(yīng)用： 比色檢測(cè)

應(yīng)用說(shuō)明： 用于定量測(cè)定培養(yǎng)物上清液、血漿、血清、唾液和尿液中任何物種的總一氧化氮。引用的樣本類型包括組織（參考文獻(xiàn)）。

物種反應(yīng)性： 物種獨(dú)立

使用/穩(wěn)定性： 將所有組分儲(chǔ)存在 4°C，硝酸鹽還原酶除外，在 -20°C 下儲(chǔ)存。

航運(yùn)： 在藍(lán)冰上運(yùn)輸

內(nèi)容： 微量滴定板，硝酸鹽還原酶，硝酸鹽還原酶儲(chǔ)存緩沖液，NADH，硝酸鹽標(biāo)準(zhǔn)品，反應(yīng)緩沖液濃縮物，格里斯試劑I，格里斯試劑II

一氧化氮(總)檢測(cè)試劑盒（ADI-917-020）特點(diǎn)：

高效-僅需50 ul稀釋樣品，節(jié)省寶貴的樣品

靈敏-檢測(cè)低至0.625 umol/L

值得信賴-在同行評(píng)審出版物中廣泛引用

快速-在40分鐘內(nèi)獲得多達(dá)80份重復(fù)樣品的結(jié)果

一氧化氮(總)檢測(cè)試劑盒部分產(chǎn)品文獻(xiàn)：

Heme-nitrosylated hemoglobin ａnd oxidative stress in women consuming combined contraceptives. Clinical application of the EPR spectroscopy: I.I. Lobysheva, et al.; Free Radic. Biol. Med. 108, 5224 (2017);

Nanoparticle mediated PPARγ gene delivery on dental implants improves osseointegration via mitochondrial biogenesis in diabetes mellitus rat model: Y. H. Lee, et al.; Nanomedicine 13, 1821 (2017);

1-Oleyl-lysophosphatidic acid (LPA) promotes polarization of BV-2 ａnd primary murine microglia towards an M1-like phenotype: I. Plastira, et al.; J. Neuroinflammation 13, 205 (2016), Application(s): iNOS activity assessment (through measuring accumulated total nitrate levels) primary microglia ａnd BV-2 cell supernatant;

Neuronal NOS Activates Spinal NADPH Oxidase 2 Contributing to Central Sigma-1 Receptor-Induced Pain Hypersensitivity in Mice: S.R. Choi, et al.; Biol. Pharm. Bull. 39, 1922 (2016);

Peroxiredoxin 6 expression is inversely correlated with nuclear factor-κB activation during Clonorchis sinensis infestation: J.H. Pak, et al.; Free Radic. Biol. Med. 99, 273 (2016), Application(s): Measuring nitrite/nitrate concentration in culture medium;

來(lái)源：https://www.amyjet.com/products/ADI-917-020.shtml