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艾美捷5-mC RNA甲基化定量分析試劑盒:一敏感彈性化
點(diǎn)擊次數(shù):0發(fā)布時(shí)間:2022/12/27 16:14:39
更新日期:2022/12/27 16:14:39
所 在 地:中國(guó)大陸
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艾美捷MethylFlash 5-mC RNA甲基化定量分析試劑盒 (Fluorometric)包含 5-mC RNA 甲基化定量實(shí)驗(yàn)所需的所有佳化試劑,可以使用任何物種的 total RNA 樣品來(lái)進(jìn)行實(shí)驗(yàn),如哺乳動(dòng)物、植物、真菌、細(xì)菌和病毒等,無(wú)論是從培養(yǎng)細(xì)胞、新鮮或冷凍的組織、血漿/血清或體液等來(lái)源皆可適用。
艾美捷MethylFlash 5-mC RNA甲基化定量分析試劑盒特點(diǎn):
快速 – 全部反應(yīng)只需2小時(shí)40分
高效 – 穩(wěn)定的品質(zhì)讓您的數(shù)據(jù)擁有高度重復(fù)性
方便 – 流程簡(jiǎn)單快速
高敏感性 – 可從200 ng的RNA樣品偵測(cè)到至低0.02%的5-mC RNA 修飾
一性 – 高一性抗體使用, 不會(huì)有其他unmethylated cytosine或 hydroxymethylated cytosine的交互辨識(shí)干擾
均一性 – 套組中皆附有positive和negative control
準(zhǔn)確性 – 佳的positive control設(shè)計(jì)可讓您的實(shí)驗(yàn)數(shù)據(jù)達(dá)到如HPLC-MS分析實(shí)驗(yàn)的效能
彈性化 – 採(cǎi)用strip-well的microplate格式, 能依照實(shí)驗(yàn)需求來(lái)進(jìn)行少量或是高通量實(shí)驗(yàn)
5-mC RNA甲基化定量分析試劑盒引文賞析:
Betlej G et. al. (October 2022). RNA 5-methylcytosine status is associated with DNMT2/TRDMT1 nuclear localization in osteosarcoma cell lines. J Bone Oncol. 36:100448.
Bataglia L et. al. (July 2021). Active genic machinery for epigenetic RNA modifications in bees. Insect Mol Biol.
Park MH et. al. (January 2020). Mono-(2-Ethylhexyl)phthalate Regulates Cholesterol Efflux via MicroRNAs Regulated m<sup>6</sup>A RNA Methylation. Chem Res Toxicol.
Lewinska A et. al. (August 2017). Reduced levels of methyltransferase DNMT2 sensitize human fibroblasts to oxidative stress and DNA damage that is accompanied by changes in proliferation-related miRNA expression. Redox Biol. 14:20-34.
Lewinska A et. al. (February 2017). Downregulation of Methyltransferase Dnmt2 Results in Condition-dependent Telomere Shortening and Senescence or Apoptosis in Mouse Fibroblasts. J Cell Physiol.