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中英文說明書丨SYSY NeuN抗體參數(shù)及應(yīng)用實例
點擊次數(shù):0發(fā)布時間:2022/9/2 17:20:45
更新日期:2022/9/2 17:20:45
所 在 地:中國大陸
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詳細內(nèi)容
Background
NeuN (Neuronal Nuclei) is a neuron-specific nuclear protein that has recently been identified as Fox-3/Rbfox3, a member of the Fox-1 family of transcription factors.
NeuN is only expressed in the nuclei of differentiated neurons. In some neurons - Purkinje cells, sympathetic ganglion cells, INL retinal cells, Cajal-Retzius cells, inferior olivary and dentate nucleus neurons - NeuN is not detectable.
1 ) Morin LP, Brain research (2011) 1421: 44-51.
2 ) Dent MA, FEBS letters (2010) 58413: 2767-71.
3 ) Cannon JR, Neuroscience letters (2009) 4641: 14-7.
4 ) Kim KK, The Journal of biological chemistry (2009) 28445: 31052-61.
5 ) Mullen RJ, Development (Cambridge, England) (1992) 1161: 201-11.
NeuN antibody - 266 004
NeuN is a neuron specific nuclear protein in vertebrates
Guinea pig polyclonal antiserum
Cat. No. 266 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery
IHC: 1 : 100 up to 1 : 500 gallery
IHC-P: 1 : 200 gallery
Immunogen Recombinant protein corresponding to AA 1 to 97 from mouse NeuN (UniProt Id: Q8BIF2)
Reactivity Reacts with: rat (D4A2H6), mouse (Q8BIF2).
Other species not tested yet.
Specificity Specific for NeuN.
Protocols
ICC
PFA fixation
IHC
Standard protocol
Tissue preparation
IHC-P
Chromogenic detection
Fluorescent detection
Tissue preparation
艾美捷SYSY NeuN抗體中文說明書:
cat#266 004;100 µl 抗血清,凍干。復溶時加入 100 µl H 2 O,然后分裝并儲存在-20°C 直至使用。
艾美捷SYSY NeuN抗體應(yīng)用:
ICC:1:500 gallery
IHC:1:100 至 1:500 gallery
IHC-P:1:200 gallery
免疫原 對應(yīng)于來自小鼠 NeuN 的 AA 1 至 97 的重組蛋白(UniProt Id:Q8BIF2)
反應(yīng)性 與以下物質(zhì)發(fā)生反應(yīng):大鼠 ( D4A2H6 )、小鼠 ( Q8BIF2 )。
其他物種尚未測試。
特異性 特定于 NeuN。
艾美捷Synaptic Systems NeuN抗體參考文獻(只展示部分):
ICC(7):
用于檢測培養(yǎng)物中神經(jīng)元信號的磁性隧道結(jié)傳感器陣列的生物相容性。
Moretti D, DiFrancesco ML, Sharma PP, Dante S, Albisetti E, Monticelli M, Bertacco R, Petti D, Baldelli P, Benfenati F
Frontiers in neuroscience (2018) 12: 909. 266 004 ICC;測試物種:大鼠
IHC(65):
表面活性劑蛋白 C 與周圍神經(jīng)網(wǎng)絡(luò)相關(guān),并顯示野生型和 3xTg 小鼠大腦內(nèi)容和海馬沉積物的年齡依賴性變化。
Schob S、Puchta J、Winter K、Michalski D、Mages B、Martens H、Emmer A、Hoffmann KT、Gaunitz F、Meinicke A、Krause M 等。
化學神經(jīng)解剖學雜志(2021)118:102036。266 004 IHC;測試物種:老鼠
圖:用抗 NeuN(稀釋 1:200;綠色)和兔抗 MBP(貨號295 002,稀釋 1:200;紅色)對石蠟包埋的小鼠腦切片進行間接免疫染色。細胞核已通過 DAPI 染色(藍色)可視化
Clariy(1):
標準化小鼠模型中不同系統(tǒng)群 I 蝙蝠狂犬病病毒的比較發(fā)病機制。
Klein A、Eggerbauer E、Potratz M、Zaeck LM、Calvelage S、Finke S、Müller T、Freuling CM
PLoS 被忽視的熱帶病 (2022) 161: e0009845。266 004 克拉利;測試物種:老鼠
來源:https://www.amyjet.com/products/266-004.shtml
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